![]() ![]() AF1q had an effect on ICAM-1 promoter region and regulated its transcription. Moreover, overexpression of AF1q attenuated ICAM-1 expression, whereas suppression of AF1q elicited the opposite effect. ![]() Likewise, we identified that ICAM-1 expression is attenuated in metastatic cells compared to primary tumor cells. Results: Most genes were directly linked with intercellular adhesion molecule-1 (ICAM-1). Methods: To investigate whether AF1q has a responsibility in the acquisition of a metastatic phenotype, we performed RNA-sequencing (RNA-Seq) to identify the gene signature and applied the Metacore direct interactions network building algorithm with the top 40 amplicons of RNA-Seq. Q: Why is the FlowJo Portal website not working once I log in?Ī: The FlowJo Portal website works best in Google Chrome, and will frequently have issues in other browsers.Aim: To investigate whether AF1q, overexpressed in metastatic cells compared with the primary tumor cells, plays a pivotal role in breast cancer metastasis. Q: Can I change the name or the computer associated with the license?Ī: Yes, contact the flow cytometry facility. Q: Why did I receive an error message when logging into FlowJo Portal account in FlowJo software?Ī: For shared computer licenses, you must be on the computer associated with the license. For individual licenses, you can only have FlowJo open on one computer at a time. You are not charged for the individual FlowJo Portal accounts. Q: Do I get billed for everyone who creates a FlowJo Portal account?Ī: You only are billed for the actual licenses that you requested. Q: Can I receive another invitation if the one I got did not work?Ī: Yes, contact the flow cytometry facility for another invitation. Q: I did not receive an invitation to create my account when will I receive it?Ī: If you requested an individual license and did not receive an invitation, contact the flow cytometry facility. If you requested a shared computer license, you will not receive an invitation. Q: Who do I contact if I am having issues with my license, FlowJo software, etc.?Ī: Contact instead of the flow cytometry facility. See our FlowJo Portal quick guide for more information.The FlowJo Portal account is not the license itself therefore creating an account will not cause you to get billed.Anyone who wants to use FlowJo must have a portal account regardless if they are using a shared computer account.Go to and then click create account on the bottom left corner of the popup.If you have not heard from the flow cytometry facility about your license, contact the facility directly.įor help with your license, contact the flow cytometry facility. It can take up to a week or two for a new license to be activated.ĭo not fill out multiple request forms for the same license. The FlowJo Portal system has a new billing system. Mac: Apple ⇒ About this Mac ⇒ More info ⇒ Network ⇒ Locations ⇒ Ethernet 1: ⇒ Hardware (MAC) Addressįill out this request form to add a new license, to move an existing license from one computer to another, to change information associated with a FlowJo license, or to cancel a FlowJo license. PC: Start ⇒ Run ⇒ CMD ⇒ ipconfig/all ⇒ Local Area ⇒ Physical Address Make sure that you are linked to a project (fund code or PO#) in your laboratory manager or financial manager of the fund code or PO# to be linked.įind your hardware address (if you are requesting a shared computer license): News and Information toggle sub nav items.Duke Consortia for HIV/AIDS Vaccine Development.Collaborative Influenza Vaccine Innovation Centers.Immunology Virology Quality Assessment Center.Collaboration for AIDS Vaccine Discovery.Programs and Centers toggle sub nav items. ![]()
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